Reporter
FG

Part:BBa_K1442106:Design

Designed by: Dan Goss   Group: iGEM14_Warwick   (2014-10-07)

Forward GFP for human cells


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 79


Design Notes

This module was designed so that different IRES’s can be tested in the same module in human cells. The GFP has been codon optimised specifically for human cells.

Source

The GFP has been obtained from NCBI and then codon optimised using the IDT codon optimisation software.

References

https://www.idtdna.com/CodonOpt